Phylogenetic Relationships and Diversity in Leymus (Poaceae, Triticeae) Based on Simple Sequence Repeats Markers

Aims: The objective of the study was to investigate: (1) Ns genome donor and elucidate origins Xm Leymus; (2) evaluate phylogenetic relationships among these species.
 Methodology: CTAB (cetyltrimethylammonium bromide) procedure used extract total genomic DNA from fresh leaf tissue. A 150 pairs SSR primers were tested screen those produced polymorphic bands continue further analysis with Elymus species 13 diploid perennial as templates. GS construct a dendrogram via unweighted pair group method arithmetic average (UPGMA) SHAN (sequential, hierarchical, agglomerative, nested clustering) routine in NTSYS-pc program.
 Results: WMC475, WMC11 QWM213 showed more expansion efficiency research. There significant diversity polymorphism between Leymus related Triticeae based on makers. largest coefficient values Pse. stipifolia hystrix indicates that genetic distance is closest has closer relationship. In clade I, formed very wellsupported into small distinct (Ia) first. Specifically, L. racemosus, salinus, secalinus triticoides clustered closely.
 Conclusion: Psathyrostachys juncea, Psa. huanshanica fragilis (Ns genome) together I species, which illustrates contains closely Psathyrostachys. St J did not participate origin Leymus, relationship are geographical distribution environment.