Photoreactivation, Excision, and Strand-Rejoining Repair in R Factor-Containing Minicells of Escherchia coli K-12
نویسندگان
چکیده
منابع مشابه
Synthesis of ribonucleic acid and protein in plasmid-containing minicells of Escherichia coli K-12.
Unlike the deoxyribonucleic acid (DNA)-deficient minicells produced by F(-) parents, minicells produced by plasmid-containing strains contain significant amounts of plasmid DNA. We examined the ability of plasmid-containing minicells to synthesize ribonucleic acid (RNA) and protein. In vivo, minicells produced by F(-) parents are unable to incorporate radioactive precursors into acid-insoluble ...
متن کاملDimer excision and repair replication patch size in recL152 mutant of Escherichia coli K-12.
Dimers are excised slowly in a recL152 mutant. This observation is not an artifact of altered DNA degradation because degradation is the same in recL+ and recL strains. The repair patch size was measured by the bromodeoxyuridine-313 nm radiation photolysis technique. In the recL+ strain, the average patch size was found to be about 30 nucleotides in length, but in the recL mutant, it was about ...
متن کاملInhibition of cell division in Escherichia coli K-12 by the R-factor R1 and copy mutants of R1.
The effect of the copy number of plasmid R1drd-19 on cell division of Escherichia coli K-12 was studied in populations growing as steady-state cultures at different growth rates, the growth rate being varied by use of different carbon sources. The plasmid copy number was also varied by using copy mutants of the R-factor. The mean cell size was larger in populations carrying an R-factor than in ...
متن کاملYield of x-ray-induced DNA single-strand breaks in niacin-starved E. coli K-12.
1. Introduction We have recently presented evidence for the existence of an ultra-fast repair process in E. coli K-12, which acts mainly on DNA single-strand breaks produced by x-irradiation under anoxic conditions (Town, Smith and Kaplan 1972). This process is not inhibited at 0°c under normal conditions, and on its completion leaves about three times as many DNA strand breaks unrepaired in ae...
متن کاملInitiation and velocity of chromosome replication in Escherichia coli B/r and K-12.
The macromolecular composition and a number of parameters affecting chromosome replication were examined over a range of exponential growth rates in two common Escherichia coli strains, B/r and K-12 AB1157. Based on improved measurements of DNA after treatment of exponential cultures with rifampin, the cell mass per chromosomal replication origin (initiation mass) and the time required to repli...
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 1972
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.110.1.71-80.1972