Photochemistry of monomethylated and permethylated bacteriorhodopsin
نویسندگان
چکیده
منابع مشابه
Resonance Raman study of the primary photochemistry of bacteriorhodopsin.
Resonance Raman multicomponent spectra of the light-adapted form of bacteriorhodopsin, bRLA568, and its first photoproduct, K628, have been obtained at liquid nitrogen temperatures. The spectra of both bRLA568 and K628 could be obtained with the known sample compositions under our irradiating conditions and computer subtraction techniques. In agreement with previous results, we find that both b...
متن کاملMonomeric and aggregated bacteriorhodopsin: Single-turnover proton transport stoichiometry and photochemistry.
The question of the basic functional transport unit of bacteriorhodopsin (BR) has been addressed by comparing the proton pumping stoichiometry as well as the photocycle kinetics of monomeric and aggregated BR in phospholipid vesicles. When time-resolved laser spectroscopy was used in combination with the optical pH-indicator pyranine, single-turnover experiments revealed approximately 0.5-0.8 a...
متن کاملParticipation of bacteriorhodopsin active-site lysine backbone in vibrations associated with retinal photochemistry.
Bacteriorhodopsin (bR) has been biosynthetically prepared with lysine deuterated at its alpha carbon (C alpha--H). The labeled membranes containing bR were investigated by difference Fourier transform infrared (FTIR) spectroscopy. It has been derived from K/bR and M/bR difference spectra (K and M are photocycle intermediates) that several bands previously assigned to the retinal chromophore are...
متن کاملProduction of a Novel N-Monomethylated Dideoxysugar
The importance of unusual deoxysugars in biology has become increasingly apparent over the past decade. Some, for example, play key roles in the physiological activities of the natural products to which they are attached. Here we describe a study of TylM1, a dimethyltransferase from Streptomyces fradiae involved in the production of dTDP-mycaminose. From this investigation, the manner in which ...
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ژورنال
عنوان ژورنال: Biophysical Journal
سال: 1988
ISSN: 0006-3495
DOI: 10.1016/s0006-3495(88)82989-8