Photochemical Activities of Spinach Chloroplasts Following Treatment with the Detergent Triton X-100
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چکیده
منابع مشابه
Photochemical Activities of Spinach Chloroplasts Following Treatment with the Detergent Triton X-100.
A nuinber of detergenits have beeni used to convert chloroplasts inlto smaller complexes for miiore intensive study of the properties of the photosynithetic apparatus (16). In all early series of experiments, Smiiith treated chloroplasts with digitonin, bile salts, sodium deoxycholate and sodium dodecyl sulfate (2325). The first 3 detergents removed and solubilized the chlorophyll wlhile the la...
متن کاملA photochemically active particle derived from chloroplasts by the action of the detergent Triton X-100.
Treatment of spinach chloroplasts with 4% Triton X-100 yields fragments which are separable by centrifugation. In the medium used (which is 4% in Triton X-100 and 0.5 M in sucrose) for the initial incubation particles sedimented by centrifugation at 144,000 X g for 1, 3, and 8 hours are relatively inactive in the partial photoreactions representative of photosynthesis. These particles contain a...
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The current study includes the screening of indigenous extracellular laccase producing fungus from the soil, identified as Phanerochaete chrysosporiumspecies.The selected strain was applied under the optimized condition for the production of laccase in the solid state fermentation. The effect of polyethylene glycol and Triton X-100 on the enhancement of enzyme activity and bi...
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Inhibition of the critical enzyme acetylcholinesterase (E.C. 3.1.1.7) with subsequent cholinergic crisis is the mechanism of acute toxicity of the organophosphorus insecticides (B. E. Mileson et al., 1998, Toxicol. Sci.41, 8-20). Consequently, measurement of acetylcholinesterase activity is important for evaluating the mammalian toxicity of this commonly used class of insecticides. While mammal...
متن کاملThe correlation of lipid release and photochemical activities in isolated spinach chloroplasts.
Purified isolated spinach chloroplasts were suspended in media o f different pH and the extracts obtained after centrifugation were analyzed for their lipid composition. The quantity of lipids present in the supernatant increased considerably by lowering the proton concentration. Under alkaline conditions more than 30°/o of the phosphoand sulfolipids were released from the thylakoid membranes. ...
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ژورنال
عنوان ژورنال: Plant Physiology
سال: 1965
ISSN: 0032-0889,1532-2548
DOI: 10.1104/pp.40.6.1269