Photo-caged 2-butene-1,4-dial as an efficient, target-specific photo-crosslinker for covalent trapping of DNA-binding proteins
نویسندگان
چکیده
Photo-irradiation of PBDA-modified DNA generates a long-lived intermediate that reacts selectively with Lys residues DNA-binding proteins, leading to higher crosslink yields than traditional photo-crosslinkers such as benzophenone and diazirine.
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Glutathione trapping to measure microsomal oxidation of furan to cis-2-butene-1,4-dial.
Furan is a liver carcinogen and toxicant. Furan is oxidized to the reactive dialdehyde, cis-2-butene-1,4-dial, by microsomal enzymes. This reactive metabolite readily reacts with glutathione nonenzymatically to form conjugates. A high-performance liquid chromatography-electrochemical method for the detection of cis-2-butene-1,4-dial-glutathione (GSH) conjugates in microsomal preparations was de...
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ژورنال
عنوان ژورنال: Chemical Science
سال: 2023
ISSN: ['2041-6520', '2041-6539']
DOI: https://doi.org/10.1039/d3sc03719c