Phosphorylation of Acid-soluble Proteins in Isolated Nucleoli of Novikoff Hepatoma Ascites Cells
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چکیده
منابع مشابه
Phosphorylation of acid-soluble proteins in isolated nucleoli of Novikoff hepatoma ascites cells. Effects of divalent cations.
Isolated nucleoli from rat Novikoff hepatoma ascites cells incorporated 32P of [T-~~P]ATP into acid-soluble nucleolar proteins in vitro in systems containing 0.25 M sucrose, 5 mM MgCL, 12.5 mM NaCl, and 0.05 M Tris-HCl buffer (pH 7.3) at 37”. Although the total uptake of 32P reached a maximum after 15 min of incubation, the uptake into some protein bands increased with longer incubation times. ...
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RNA taken up into the cells. The 4 types of RNA used included whole cellular 4 5, 18 5, and 28 S RNA from NHAC and the very GC-rich B3 fragment enzymatically cleaved from 28 S RNA of NHAC (1 7, 37, 37). The B3 fragment, composed of approximately 550 nucleotides, was chosen because it is highly hydrogen bonded and is less susceptible to degradation by RNase. The present results show that the cel...
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The U-l RNA is one of the low molecular weight nuclear RNAs of Novikoff hepatoma ascites cells that is specifically localized to the extranucleolar portion of the nucleus. After the RNA was purified by polyacrylamide gel electrophoresis, it was subjected to various enzymatic digestion procedures for determination of its primary nucleotide sequence. The U-l RNA contains 171 nucleotides. Its 5’ e...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1974
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(20)79767-x