Phosphorylation Impacts Cu(II) Binding by ATCUN Motifs

ATCUN (amino terminal Cu(II) and Ni(II) binding) motifs chelate ions strongly. However, the impact of phosphorylation neighboring residues on such complexation has not been elucidated. The copper(II) dissociation constants original phosphorylated peptides from human histatin-1 serum albumin were compared using spectroscopic methods. Phosphorylation markedly weakened binding. Thus, these results indicate that may be a vital mechanism governing metal ion