Phosphorylation-Dependent Mobility Shift of Proteins on SDS-PAGE is Due to Decreased Binding of SDS

DNA mobility shift assay coupled with SDS-PAGE for detection of DNA-binding proteins.

DNA mobility shift assay is widely performed to analyze sequence-specific DNA-binding proteins in cellular extracts (2), and the DNA-binding proteins can be identified by use of antibodies that induce further retardation of probes (supershift). Gel extracts from a shifted band contain not only DNA segments and DNA-binding proteins but also nonspecific proteins located at the same position in th...

Study on pattern of Neospora caninumtachyzoite proteins by SDS-PAGE and Western blotting in aborted cows

The intracellular parasite Neospora caninumis prevalent in several countries and is increasingly recognized as an important cause of abortion and stillbirth in cattle. For characterizeing the tachyzoite antigens of Neospora caninumin aborted cows, sera were obtained from 116 cows which were aborted in the third semester of the pregnancy period and had antibodies to Neospora caninumin ELISA. To ...

Tricine – SDS - PAGE Hermann Schägger

Tricine–SDS-PAGE is commonly used to separate proteins in the mass range 1–100 kDa. It is the preferred electrophoretic system for the resolution of proteins smaller than 30 kDa. The concentrations of acrylamide used in the gels are lower than in other electrophoretic systems. These lower concentrations facilitate electroblotting, which is particularly crucial for hydrophobic proteins. Tricine–...

Multiple SDS-PAGE on Vertical Electrophoresis Units.

INTRODUCTION SDS-polyacrylamide gel electrophoresis (SDS-PAGE) represents the second-dimension separation of two-dimensional (2D)-PAGE. Large-scale proteome analysis usually requires simultaneous electrophoresis of batches of second-dimension SDS-PAGE gels to maximize the reproducibility of 2D electrophoresis protein profiles. This requirement is most easily met using multiple, vertical second-...

Identification of electrophoretic proteins pattern of Fusaium verticillioides isolates from maize by SDS-PAGE.