Phosphatidylcholine Synthesis in Agrobacterium tumefaciens
نویسندگان
چکیده
منابع مشابه
Virulence of Agrobacterium tumefaciens requires phosphatidylcholine in the bacterial membrane.
Phosphatidylcholine (PC, lecithin) has long been considered a solely eukaryotic membrane lipid. Only a minority of all bacteria is able to synthesize PC. The plant-transforming bacterium Agrobacterium tumefaciens encodes two potential PC forming enzymes, a phospholipid N-methyltransferase (PmtA) and a PC synthase (Pcs). We show that PC biosynthesis and tumour formation on Kalanchoë plants was i...
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Phosphatidylcholine (PC), or lecithin, is the major phospholipid in eukaryotic membranes, whereas only 10% of all bacteria are predicted to synthesize PC. In Rhizobiaceae, including the phytopathogenic bacterium Agrobacterium tumefaciens, PC is essential for the establishment of a successful host-microbe interaction. A. tumefaciens produces PC via two alternative pathways, the methylation pathw...
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Zinc and copper ions at 1.0 mM effectively inhibited synthesis of 3-ketodisaccharides (3-KD) by washed cells of Agrobacterium tumefaciens. Cu2+ at 0.1 mM completely inactivated cell·free preparations of the synthetic enzyme, hexopyranoside: cytochrome c oxidoreduc· tase. This ion also inhibited uptake of disaccharides. Since Zn2+ blocked disaccharide uptake but had no effect on the synthetic en...
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Two pantothenate-requiring mutants of Agrobacterium tumefaciens have been isolated. One of them (strain WMP-1) is unusual in that growth levels equivalent to the parent strain are achieved only when the medium is additionally supplemented with aspartate or another compound related to the tricarboxylic acid cycle. Extracts of cells grown on limiting aspartate were found to contain four times mor...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1964
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)91245-7