Phosphate-binding sequences in nucleotide-binding proteins

Allosteric determinants in guanine nucleotide-binding proteins.

Members of the G protein superfamily contain nucleotide-dependent switches that dictate the specificity of their interactions with binding partners. Using a sequence-based method termed statistical coupling analysis (SCA), we have attempted to identify the allosteric core of these proteins, the network of amino acid residues that couples the domains responsible for nucleotide binding and protei...

5'-Phosphate-Binding Sites in Proteins

many enzymic reactions. Almost all these pyridoxal phosphate-dependent enzymes have an absorption band around 415nm, although most of them have higher peaks at 335 nm. The studies made with simple models have shown that the 415nm band is due to a Schiffbase in a hydrophilic medium [structure (I) of Scheme 1] (Matsushima & Martell, 1967). The 335nm band can be assigned either to the structure (I...

Short nucleotide sequences signal spliceosomal binding in nucleic acids.

We have explored the region around the splice sites of the human intron and exons from the exon-intron database (EID) and located a number of short 6-nucleotide and 7-nucleotide sequences that are relatively common in the regions. These short sequences, we expect play an important role in the selection of the appropriate splicing process. We propose that the external signals via short recogniti...

آنالیز پروفایل nbs (nucleotide binding-site) ژنهای مقاومت به بیماری در ارقام مختلف زیتون

به منظور مطالعه ژنهای مقاومت nbs-lrr و تنوع ژنتیکی آنها در بین ارقام بومی زیتون و همچنین مقایسه آن با ارقام خارجی، از تکنیک profiling nbs و آنالیزهای مولکولی استفاده شد. برای این منظور dna ژنومی تعداد 5 رقم خارجی و 5 رقم داخلی زیتون استخراج و با آنزیم های برشی alui و rsai هضم و با استفاده از 3 آغازگر دژنره( nbs2،nbs7،nbs5a) در دو مرحله تکثیر شدند و الگوی باندی حاصل از تکثیر انتخابی آنها در روی ...

Nucleotide binding