Phloroglucinol Improves Direct Rooting of In Vitro Cultured Apple Rootstocks M9 and M26

Factors affecting induction and development of in vitro rooting in apple rootstocks.

Shoots of apple rootstocks raised in vitro were transferred to various rooting media to study the effect of different factors on root initiation and development. Various concentrations of indole-3-butyric acid (IBA) initiated rooting but maximum rooting percentage was found with 2.0 and 2.5 mg l(-1) of IBA in M7 and with 1.0 mg l(-1) of IBA in MM106. The drawback was that the roots were thick, ...

Impacts of plant growth regulators and culture media on in vitro propagation of three apple (Malus domestica Borkh.) rootstocks

Apple is one of the most important temperate-zone fruit trees in the world. Micropropagation of apple scion and rootstocks is an important method of rapid production of healthy and disease-free plants. In this research, in vitro propagation of three apple roottocks including Azayesh-Esfahan, Morabbaee-Mashhad and M9 was investigated. Shoot proliferation carried out in two basal media (MS and WP...

Successful propagation in vitro of apple rootstock MM106 and influence of phloroglucinol.

Successful in vitro propagation of clonal apple rootstock MM106 was achieved by culturing axillary buds on MS basal medium with BAP (1 mg/L), GA3 (0.5 mg/L) and IBA (0.1 mg/L). Use of liquid medium (LM) in initial cultures reduced phenol exudation to a greater extent and gave maximum sprouting percentage when transferred to solid MS medium. Phloroglucinol (PG) did not enhance sprouting of buds ...

Rootstocks for Size Control in Apple Trees

Effects of Salinity on In vitro Shoot Proliferation and Rooting of Apple Rootstock MM.106

In the present study, the in vitro response of MM.106 apple rootstock to increasing concentrations of NaCl (0, 20, 40, 80, 100 and 120 mM) in the Murashige and Skoog culture medium was analyzed. Explant growth was seriously affected by salinity treatments. Elevated salinity from 20 (control) to 40, 80, 100 and 120 mM NaCl resulted in reduction in shoot growth (shoot number, length and fresh wei...