Phenotypic Restoration by Molybdate of Nitrate Reductase Activity in chlD Mutants of Escherichia coli
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چکیده
منابع مشابه
Phenotypic Restoration by Molybdate of Nitrate
ChlD mutants of Escherichia coli are pleiotropic, lacking formate-nitrate reductase activity as well as formate-hydrogenlyase activity. Whole-chain formate-nitrate reductase activity, assayed with formate as the electron donor and measuring the amount of nitrite produced, was restored to wild-type levels in the mutants by addition of 10-4 M molybdate to the growth medium. Under these conditions...
متن کاملchlD gene function in molybdate activation of nitrate reductase.
chlD mutants of Escherichia coli lack active nitrate reductase but form normal levels of this enzyme when the medium is supplemented with 10-3 M molybdate. When chlD mutants were grown in unsupplemented medium and then incubated with molybdate in the presence of chloramphenicol, they formed about 5% the normal level of nitrate reductase. Some chlD mutants or the wild type grown in medium supple...
متن کاملThe molybdate-responsive Escherichia coli ModE transcriptional regulator coordinates periplasmic nitrate reductase (napFDAGHBC) operon expression with nitrate and molybdate availability.
Expression of the Escherichia coli napFDAGHBC operon (also known as aeg46.5), which encodes the periplasmic molybdoenzyme for nitrate reduction, is increased in response to anaerobiosis and further stimulated by the addition of nitrate or to a lesser extent by nitrite to the cell culture medium. These changes are mediated by the transcription factors Fnr and NarP, respectively. Utilizing a napF...
متن کاملAnalysis of nitrate reductase mRNA expression and nitrate reductase activity in response to nitrogen supply
Nitrate is one of the major sources of nitrogen for the growth of plants. It is taken up by plant roots and transported to the leaves where it is reduced to nitrite in the. The main objective of this research was to investigate stimulatory effects of sodium nitrate, potassium nitrate, ammonia and urea on the production/generation of the nitrate reductase mRNA in Triticum aestivum plants. The pl...
متن کاملThe induced formation of nitrate reductase in auxotrophic mutants of Escherichia coli.
enzymes in the absence of the factor required for growth (Monod, Pappenheimer and CohenBazire, Biochem. et Biophys. Acta, 6, 468, 1951; Pardee, Proc. Natl. Acad. Sci. U. S., 40, 263, 1954; J. Bacteriol., 69, 233, 1955), but a thyminerequiring strain can synthesize inducible xylose isomerase enzyme in the absence of thymine (Cohen and Barner, Proc. Natl. Acad. Sci. U. S., 40, 263, 1954). Formati...
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 1971
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.108.2.854-860.1971