Phenotypic Characterization and Prevalence of Carbapenemase-Producing Pseudomonas aeruginosa Isolates in Six Health Facilities in Cameroon

Pseudomonas aeruginosa is a Gram-negative opportunistic pathogen with great ability to adapt stress, in particular, the selective pressure of antibiotics hospital environment. This constitutes real public health concern, especially low- and middle-income countries. In Cameroon, little known about drug resistance patterns aeruginosa. study sought determine prevalence strains producing carbapenemases six facilities center, littoral, west regions Cameroon. An analytical cross-sectional was conducted over four-month period from July October 2021. All or suspected isolated pathological products at bacteriology laboratory different were systematically collected underwent re-identification. After growing on cetrimide agar successfully subculturing nutrient agar, an oxidase test performed pure colonies, followed by biochemical identification (API 20NE system) bacterial suspension (0.5McFarland standard). Drug susceptibility testing for detection extended-spectrum beta-lactamases overproduced inducible cephalosporinases according adequate standard procedures. Of 468 isolates collected, 347 (74.14%) confirmed after re-identification, which 34.49% (120/347) produced (CAZR C/TS) 32.26% (112/347) beta-lactamases. The carbapenemase-producing P. (IMPR C/TR) 25.07% (87/347), 17.24% (15/87) class A 82.76% (72/87) B. high rate penicillin (piperacillin: 70.58% ticarcillin: 60.24%) observed. We also noted ceftazidime, 30.22% imipenem against 37.02% meropenem, 25.1% ceftolozane/tazobactam (C/T). These exhibited 79.57% quinolones 26% aminoglycoside families. Multivariate analysis revealed that aeruginosa-related infections significantly associated hospitalization (p = 0.04), maternity 0.03), surgery intensive care wards 0.04). highlighted burden resistant Surveillance should be intensified prevent dissemination spread these strains.