Phenotype characteristic of colonic intraepithelial lymphocytes in patients with Crohn's disease

Intraepithelial lymphocytes (IEL) play a critical role in maintaining the immune balance of gut and provide first line mucosal defense against luminal antigens as well rapidly respond to epithelial injury. Recently, IEL have received lot attention key mediators aberrant response resulted persistent activation, inflammation altered intestinal barrier function, seen Crohn's disease (CD). This study describes for time subsets colonic CD patients compared healthy controls aimed at characterization contribution pathogenesis disease. The peripheral venous blood colon tissues were obtained from 10 6 donors. isolated mucosa by incubation tissue predigesting solution. Lymphoid cells phenotype was investigated using monoclonal antibodies flow cytometry. majority identified СD3 + T no significant differences found their numbers groups. However, changes cell composition been shown: ratio СD4 СD8 1:1 correlated with (R = 0.7; p < 0.05) while donor characterized expected killers prevalence reached 1:2 (p 0.05). increase unconventional γδIEL (mainly due V81+T cells) СD161 association TNK decrease revealed 0.01) Moreover, number location -0.6; 0.05), behavior according Montreal classification. observed data indicates that may valuable insight into helpers, γδT mucosa-associated autoimmune but need further possible mechanisms discussion.