pH regulated lactose inspired fabrication of zinc oxide nanoparticles for insulin sensing by LSPR absorption

Nanostructured metal oxide particles with diversified morphologies are in high demand nanotechnology. The particle size, shape, and overall geometry mainly depend on the fabrication method. This study reports synthesis of zinc nanoparticles (ZnO NPs) from nitrate hexahydrate [Zn(NO3)2.6H2O] precursor aqueous media at 65 °C by using lactose cow milk as a reducing agent regulating pH 6 to 10. UV–visible absorption gave maximum absorbance (λmax) 371–375 nm ethanol for localized surface plasmon resonance (LSPR), FTIR exhibited bands ca. 439–481 cm−1 stretching mode Zn–O bonds, XRD peaks 2 θ values 31.8, 34.45, 36.28° confirmed fabricated ZnO NPs. spectra also indicated that crystallite (20–30 nm) has hexagonal wurtzite structure. average sizes measured DLS were 50–837 nm, SEM microphotographs demonstrated morphology NPs hexagonal, rod-shaped, or spike-like used investigate LSPR various concentrations insulin, ranging 2.5 μM 50 μM. 7 10 showed better insulin sensing performance precision. approach variable would play significant function biomedical science especially real time monitoring glucose efficient management diabetes.