pH-induced structural changes in human serum apotransferrin pKa values of histidine residues and N-terminal amino group determined by 1H-NMR spectroscopy
نویسندگان
چکیده
منابع مشابه
Determination of the pKa of the N-terminal amino group of ubiquitin by NMR
Ubiquitination regulates nearly every aspect of cellular life. It is catalysed by a cascade of three enzymes and results in the attachment of the C-terminal carboxylate of ubiquitin to a lysine side chain in the protein substrate. Chain extension occurs via addition of subsequent ubiquitin molecules to either one of the seven lysine residues of ubiquitin, or via its N-terminal α-amino group to ...
متن کاملPrecise limits of the N-terminal domain of DnaB helicase determined by NMR spectroscopy.
Two separate N-terminal fragments of the 470-amino-acid Escherichia coli DnaB helicase, comprising residues 1-142 and 1-161, were expressed in E. coli. The proteins were extracted in a soluble fraction, purified, and characterised physically. In contrast to the full-length protein, which is hexameric, both fragments exist as monomers in solution, as demonstrated by sedimentation equilibrium mea...
متن کاملN-terminal amino acid sequence of wheat proteins that lack phenylalanine and histidine residues.
The 24 residues of the N-terminal CNBr peptide from a wheat albumin, that lacks phenylalanine and histidine, have been sequenced. Three of the assignments were made partly by analogy with two other proteins, as evidence is presented that all three proteins are probably identical in this region. Extra evidence for the sequences of the alpha-chymotryptic peptides derived from the N-terminal CNBr ...
متن کاملMonitoring of dissolution induced changes in film coat composition by 1H NMR spectroscopy and SEM.
In our study we investigated the release mechanisms of coated oral dosage forms more deeply. The aim of the study was to monitor the buffer induced leaching out of water soluble compounds from Kollicoat SR films and to relate this process to the film properties and the release kinetics. Propranolol HCl tablets were coated with different amounts and ratios of polyvinyl acetate (Kollicoat SR) and...
متن کاملN-Terminal Amino Acid Residues of Gelatin
Levvy, G. A. (1948). Biochem. J. 42, 2. Levvy, G. A. (1952). Biochem. J. 52, 464. Levvy, G. A. (1954). Biochem. J. 58, 462. Levvy, G. A. & Marsh, C. A. (1952). Biochem. J. 52, 690. Lohmar, R., Dimler, R. J., Moore, S. & Link, K. P. (1942). J. biol. Chem. 143, 551. Marsh, C. A. (1952). J. chem. Soc. p. 1578. Marsh, C. A. (1954). Biochem. J. 58, 609. Marsh, C. A., Alexander, F. & Levvy, G. A. (19...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: European Journal of Biochemistry
سال: 1994
ISSN: 0014-2956,1432-1033
DOI: 10.1111/j.1432-1033.1994.tb18679.x