Perspective of nucleic acid electrophoresis.

Principles of Nucleic Acid Separation by Agarose Gel Electrophoresis

Agarose gel electrophoresis is a routinely used method for separating proteins, DNA or RNA. (Kryndushkin et al., 2003). Nucleic acid molecules are size separated by the aid of an electric field where negatively charged molecules migrate toward anode (positive) pole. The migration flow is determined solely by the molecular weight where small weight molecules migrate faster than larger ones (Samb...

The nucleic acid of African Horse-Sickness Virus

Isolation of the Polysaccharides and Nucleic Acid of Tuberculin by Electrophoresis”

The source of this material was the filtrate obtained after a protein fraction had been removed by precipitation with ammonium sulfate. The scheme of chemical fractionation was as follows: Human strain tubercle bacilli (DT) from the Bureau of Animal Industry, United States Department of Agriculture, were grown in liter bottles on Long’s synthetic medium for 8 to 10 weeks, at 37”; 3664 cultures ...

Computer programs for analysis of nucleic acid hybridization, thermal denaturation, and gel electrophoresis data.

Computer programs for the analysis of data from techniques frequently used in nucleic acids research are described. In addition to calculating non-linear, least-squares solutions to equations describing these systems, the programs allow for data editing, normalization, plotting and storage, and are flexible and simple to use. Typical applications of the programs are described.

Molecular Beacons of Xeno-Nucleic Acid for Detecting Nucleic Acid

Molecular beacons (MBs) of DNA and RNA have aroused increasing interest because they allow a continuous readout, excellent spatial and temporal resolution to observe in real time. This kind of dual-labeled oligonucleotide probes can differentiate between bound and unbound DNA/RNA in homogenous hybridization with a high signal-to-background ratio in living cells. This review briefly summarizes t...