Peritoneal macrophage activation indicated by enhanced chemiluminescence
نویسندگان
چکیده
منابع مشابه
Proteolytic cleavage and activation of pro-macrophage-stimulating protein by resident peritoneal macrophage membrane proteases.
Macrophage stimulating protein (MSP), which is secreted as biologically inactive pro-MSP, is activated to MSP by cleavage at a single peptide bond. Our objectives were to determine the form of MSP in circulating blood and to study proteolytic activation of pro-MSP by its target cell. Western blot of immunoaffinity-purified serum MSP showed that all the protein was pro-MSP, without detectable MS...
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Action of poly-[(trans-l,2-diaminocyclohexane)platinum]-carboxyamylose (poly-plat) and cisplatin (CDDP) on the immune system was studied using isolated peritoneal macrophages and compared with liver resident macrophages from normal, CDDP and poly-plat [10 mg/kg] treated Swiss Webster mice for 2-12 days. Peritoneal macrophages were treated [10 mg/L] for 2 h and allowed to grow in normal medium a...
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The significant enhancement of acidic potassium permanganate chemiluminescence by Mn(II) results from the concomitant presence of permanganate and Mn(III) in the reagent solution, which enables rapid production of the excited Mn(II) emitter with a wide range of analytes. Furthermore, the key Mn(III) co-reactant can be quickly generated by reducing permanganate with sodium thiosulfate, instead o...
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Activation of macrophages may be one of the possible approaches in modulating inflammation. We previously reported that Bougainvillea xbuttiana extract showed an immunomodulatory activity. Here we compare the activation of macrophages exposed to B. xbuttiana extract and compare it with the other treatments such as LPS, IL-4, and IL-10. The cytotoxic effect of extract on peritoneal macrophages w...
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To investigate the effects of peritoneal fluid from patients with endometriosis on mouse peritoneal macrophages (Mphi), peritoneal fluid from endometriosis patients (n = 15) were added to a monolayer of C3H/HeJ mouse peritoneal Mphi. Tumor necrosis factor-producing activity was measured by the L929 assay activated with FK-23 (a preparation of heat-killed Enterococcus faecalis). Tumor necrosis f...
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ژورنال
عنوان ژورنال: Infection and Immunity
سال: 1978
ISSN: 0019-9567,1098-5522
DOI: 10.1128/iai.21.3.886-895.1978