Pentavalent Arsenate Transport by Zebrafish Phosphate Transporter NaPi-IIb1
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چکیده
منابع مشابه
Regulation of phosphate (Pi) transport and NaPi-III transporter (Pit-1) mRNA in rat osteoblasts.
In osteoblasts only the type III Na(+)-dependent phosphate (NaPi) transporter isoforms Pit-1 and Pit-2 have been identified. We tested the effects of extracellular Pi, Ca(2+) and IGF-I on Na(d)Pi transport and Pit-1 or Pit-2 mRNA expression in rat osteoblastic (PyMS) cells. The v(max) of Na(d)Pi transport was higher in cells kept in Pi-free, serum-free medium for 24 h than in controls at 1 mM P...
متن کاملUp-Regulation of Intestinal Phosphate Transporter NaPi-IIb (SLC34A2) by the Kinases SPAK and OSR1.
BACKGROUND/AIMS SPAK (SPS1-related proline/alanine-rich kinase) and OSR1 (oxidative stress-responsive kinase 1), kinases controlled by WNK (with-no-K[Lys] kinase), are powerful regulators of cellular ion transport and blood pressure. Observations in gene-targeted mice disclosed an impact of SPAK/OSR1 on phosphate metabolism. The present study thus tested whether SPAK and/or OSR1 contributes to ...
متن کاملDown-regulation of the Na+-coupled phosphate transporter NaPi-IIa by AMP-activated protein kinase.
BACKGROUND/AIMS The Na(+)-coupled phosphate transporter NaPi-IIa is the main carrier accomplishing renal tubular phosphate reabsorption. It is driven by the electrochemical Na(+) gradient across the apical cell membrane, which is maintained by Na(+) extrusion across the basolateral cell membrane through the Na(+)/K(+) ATPase. The operation of NaPi-IIa thus requires energy in order to avoid cell...
متن کاملCharacterization of a type IIb sodium-phosphate cotransporter from zebrafish (Danio rerio) kidney.
Zebrafish (Danio rerio) express two isoforms of the type IIb Na-dependent P(i) cotransporter (NaPi). Type NaPi-IIb1 has previously been cloned and characterized. Here, we report the cloning of the NaPi-IIb2 transcript from zebrafish kidney, its localization, and its functional characterization. RT-PCR with renal RNA and degenerate NaPi-IIb-specific primers resulted in a specific fragment. 3'-Ra...
متن کاملPhosphate transport by the human renal cotransporter NaPi-3 expressed in HEK-293 cells.
The human renal Na-PO4cotransporter gene NaPi-3 was expressed in human embryonic kidney HEK-293 cells, and the transport characteristics were measured in cells transfected with a vector containing NaPi-3 or with the vector alone (sham transfected). The initial rate of32PO4influx had saturation kinetics for external Na and PO4 with[Formula: see text] of 128 mM (PO4 = 0.1 mM) and[Formula: see tex...
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ژورنال
عنوان ژورنال: Zebrafish
سال: 2011
ISSN: 1545-8547,1557-8542
DOI: 10.1089/zeb.2011.0701