PCR versus Immunohistochemistry for Microsatellite Instability
نویسندگان
چکیده
منابع مشابه
Usefulness of Immunohistochemistry for Microsatellite Instability Screening in Gastric Cancer
BACKGROUND/AIMS The usefulness of immunohistochemistry to screen for the microsatellite instability (MSI) phenotype in gastric cancer remains unclear. Moreover, the prognostic value of MSI phenotypes in gastric cancer has been debated. METHODS The clinicopathologic parameters and survival outcomes of 203 MSI-high (MSI-H) and 261 microsatellite-stable (MSS) advanced gastric cancers (AGCs) were...
متن کامل[Lynch syndrome: selection of families by microsatellite instability and immunohistochemistry].
BACKGROUND Selection of patients with Lynch Syndrome (LS) for a genetic study involves the application of clinical criteria. To increase the rate of identification of mutations, the use of molecular studies as Microsatellite Instability (MSI) and Immunohistochemistry (IHC) in the tumor has been proposed. AIM To demonstrate the usefulness of MSI and IHC in the detection of mutations in patient...
متن کاملReflex microsatellite instability & immunohistochemistry testing practices & follow-up of abnormal results at U.S. cancer programs
Background Abnormal results of microsatellite instability (MSI) or immunohistochemistry (IHC) testing of colorectal (CRC) tumors may suggest the presence of Lynch Syndrome (LS), a hereditary cause of CRC. Individuals with LS are at high risk for second primary cancers. Abnormal results of MSI and IHC testing influence care decisions. MSI is also a prognostic marker. The purpose of this survey-b...
متن کاملImmunohistochemistry and microsatellite instability analysis in molecular subtyping of colorectal carcinoma based on mismatch repair competency.
Mismatch repair defective (MMRd) colorectal carcinoma (CRC) is a distinct molecular phenotype of colorectal cancer, including 12% of sporadic CRC and 3% of Lynch Syndrome. In order to investigate the clinicopathological characteristics of MMRd colorectal carcinoma, and to find the most effective method for preliminary screening, 296 CRC fulfilled revised Bethesda Guideline (RB) were selected fr...
متن کاملMicrosatellite instability, immunohistochemistry, and additional PMS2 staining in suspected hereditary nonpolyposis colorectal cancer.
PURPOSE Immunohistochemistry (IHC) and microsatellite instability (MSI) analysis can be used to identify patients with a possible DNA mismatch repair defect [hereditary nonpolyposis colorectal carcinoma (HNPCC)]. The Bethesda criteria have been proposed to select families for determination of MSI. The aims of this study were to assess the yield of MSI analysis in families suspected for HNPCC, t...
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ژورنال
عنوان ژورنال: The Journal of Molecular Diagnostics
سال: 2008
ISSN: 1525-1578
DOI: 10.2353/jmoldx.2008.070147