PCR-SSCP and <i>Hin6</i>I PCR-RFLP detecting novel anonymous SNPs within ovine <i>PROP1</i> gene (Brief report)
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A novel EcoRII PCR-RFLP detecting genetic variation of goat NF1-C2 gene and its association with milk yield (Brief Report)
The transcription factor nuclear factor1-C2 (NF1-C2) mediates the action of prolactin in the mammary gland. Research on the molecular genetic mechanism of model system have indicated that the NF1-C2 gene plays an important role for the activation of several mammary gland specific genes (Nilsson et al. 2006). Moreover, the effects of prolactin on milk production traits have been reported in rumi...
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BACKGROUND AND OBJECTIVES Resistance in Mycobacterium tuberculosis is caused by mutations in genes encoding drug targets. Investigators have already demonstrated the existence of mutations in codons 88 to 94 in the gyrA gene and also in codons 1400, 1401, and 1483 of rrs gene among extensively and extremely drug resistant tuberculosis (XDR & XXDR-TB) strains. The aim of this study was to identi...
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The genotypes for calpastatin (CAST) and calpain (CAPN) loci were determined by PCR-RFLP and PCRSSCPmethods. Blood samples were collected from 200 pure-bred Zel sheep from Shirang’s Zel sheep Breeding Station located in south-west of Golestan, Iran. Extraction of genomic DNA was performed based on the modified salting out method. Based on results, two investigated loci were polymorphic an...
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DNA-based molecular markers for differentiation of five penaeid shrimps (Penaeus monodon, P. semisulcatus, Feneropenaeus merguiensis, Litopenaeus vannamei and Marsupenaeus japonicus) were developed based on polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and single-stranded conformation polymorphism (SSCP) of 16S ribosomal (r) DNA. Differentiation of P. monodon, P....
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The aim of present study was to investigate myostatin gene polymorphism and its association with yearling weight records in Zel sheep using PCR-RFLP and PCR-SSCP methods. Blood samples were collected from 200 Zel sheep, randomly, and DNA was extracted using modified salting out method. Polymerase chain reaction was carried out to amplify 337, 222, and 311 bp fragments, respectively, comprising ...
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ژورنال
عنوان ژورنال: Archives Animal Breeding
سال: 2008
ISSN: 2363-9822
DOI: 10.5194/aab-51-622-2008