PCR ribotyping for characterizing Salmonella isolates of different serotypes
نویسندگان
چکیده
منابع مشابه
Use of ribotyping for characterization of Salmonella serotypes.
Forty-five isolates of Salmonella serotype reading, 20 isolates of Salmonella serotype senftenberg, and 56 isolates of Salmonella serotype typhimurium from domestic and wild animals were characterized genotypically to differentiate within serotypes for epidemiologic studies. The genotypic method of characterization used was ribotyping, a method for highlighting highly conserved rRNA genes and a...
متن کاملIdentification and characterization of Salmonella isolates by automated ribotyping.
A study was conducted with the RiboPrinter, an automated ribotyping system, to evaluate its ability to identify and characterize isolates of Salmonella from broiler operations. Isolates of Salmonella obtained from a local broiler company were serotyped by a reference laboratory and ribotyped using the RiboPrinter. The RiboPrinter generated ribotype patterns by probing EcoRI digests of Salmonell...
متن کاملMolecular Analysis of Toxigenic Clostridium difficile Isolates from Hospital Environment by PCR Ribotyping Method
Background and Aims: Clostridium difficile is an identified cause of antibiotic-associated diarrhea, antibiotic-associated colitis, pseudomembranous colitis and nosocomial diarrhea. The objective of this survey was to determine molecular analysis of toxigenic Clostridium difficile isolates from hospital environment in Tehran tertiary medical centers. Materials and Methods: In this descriptiv...
متن کاملA comparative evaluation of PCR ribotyping and ERIC PCR for determining the diversity of clinical Pseudomonas aeruginosa isolates.
Two typing methods were evaluated, utilizing 62 clinical strains of Pseudomonas aeruginosa, to assess their usefulness as tools to study the bacterial diversity within this complex group. Genetic diversity was determined by PCR ribotyping and enterobacterial repetitive intergenic consensus (ERIC) PCR. By these methods, 9 and 36 genotypes were found, respectively. The result showed that ERIC PCR...
متن کاملبررسی قدرت تمایز سروتیپ‌های سالمونلا با تکنیک PCR-Ribotyping در سویه‌های جدا شده از کودکان مبتلا به اسهال زیر 15 سال
Background & Aim: Salmonella is the most important pathogenic microorganism causing food borne disease and diarrhea in humans. It could present as diarrhea, fever, vomiting and sometimes bloody diarrhea. Because of its' importance as one of the pathogens, it is essential to identify and characterize its serotypes by molecular methods. The aim of this study was to characterize PCR-Ribotyping...
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ژورنال
عنوان ژورنال: Journal of Clinical Microbiology
سال: 1996
ISSN: 0095-1137,1098-660X
DOI: 10.1128/jcm.34.10.2440-2443.1996