PCR-RFLP Based Method for Molecular Differentiation of Sand Fly Species <I>Phlebotomus argentipes</I>, <I>Phlebotomus papatasi</I>, and <I>Sergentomyia babu</I> Found in India
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منابع مشابه
A Consistent PCR-RFLP Assay Based on ITS-2 Ribosomal DNA for Differentiation of Fasciola Species
Objective(s): Fascioliasis is a zoonotic parasitic disease caused by liver fluke species of Fasciola hepatica and Fasciola gigantica. Differentiation of these two species, based on their morphological characteristics, is difficult. The current study aimed to use PCR-RFLP assay to distinguish between F. hepatica and F. gigantica , based on profiles of RFLP, produced by effect of endo...
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Background and Purpose: The Fusarium species are among the most important fungi in the medical, veterinary and agricultural fields. Materials and Methods: In the present study, 172 strains of these fungi have been analyzed. The high molecular weight DNAs were extracted from 23 reference strains as well as from 149 isolated Fusarium species. Using the designed nucleotide primers from rDNA of Fus...
متن کاملApplication of RFLP-PCR-Based Identification for Sand Fly Surveillance in an Area Endemic for Kala-Azar in Mymensingh, Bangladesh
Mymensingh is the most endemic district for kala-azar in Bangladesh. Phlebotomus argentipes remains the only known vector although a number of sand fly species are prevalent in this area. Genotyping of sand flies distributed in a VL endemic area was developed by a PCR and restriction-fragment-length polymorphism (RFLP) of 18S rRNA gene of sand fly species. Using the RFLP-PCR analysis with AfaI ...
متن کاملDifferentiation of Adulterated Meat Products through Molecular Technique: PCR-RFLP
Meat adulterations of different species are undetectable and it is common practice globally. In the field of food analysis, species determination is mostly sufficient, but simultaneous detection of several species in a single food product is desirable. The aim of the study was to distinguish between meats of two different species through PCR-RFLP analysis. The meat of two species were used incl...
متن کاملA Consistent PCR-RFLP Assay Based on ITS-2 Ribosomal DNA for Differentiation of Fasciola Species
OBJECTIVE(S) Fascioliasis is a zoonotic parasitic disease caused by liver fluke species of Fasciola hepatica and Fasciola gigantica. Differentiation of these two species, based on their morphological characteristics, is difficult. The current study aimed to use PCR-RFLP assay to distinguish between F. hepatica and F. gigantica, based on profiles of RFLP, produced by effect of endonucleases on I...
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ژورنال
عنوان ژورنال: Journal of Medical Entomology
سال: 2012
ISSN: 0022-2585,0022-2585
DOI: 10.1603/me12033