PCR-RFLP Based genetic diversity of Plasmodium vivax genotypes in district Mardan, Pakistan

Abstract Plasmodium vivax is the most common human malaria parasite in Asian countries including Pakistan. Present study was designed to explore genetic diversity of plasmodium genotypes based on Pvmsp-3α and Pvmsp-3βgenes using allelic specific nested PCR RFLP assays markers from field isolates district Mardan, Blood samples 200 P. malarial patients were collected after taking their written informed consent. Genetic products determined by Restriction Fragment Length Polymorphism (RFLP) utilizing Alu1 PstI restriction enzymes for alpha beta gene digestion, respectively. For analysis sub variants Pvmsp3α Pvmsp3β genes, Chi-Square test performed Minitab programming software 18. The P value 0.05 considered as statistically significant. genes gel electrophoresis digested products, four distinct obtained total 50 samples; type A: 35 (70%) (1.5-2.0 kb), 12 B (24%) (1.5-1.7 2 C (4%) (0.5-1.5) one D (2%) (0.5-0.65 kb) which could be characterized into 9 pattern (A1-A4, B1-B3, C1, D), A3 remained predominant. Pvmsp-3βgenes, three 40(80%) A (1.5-2.5 (18%) (1.0-1.5kb) 1(2%) (0.65 6 patterns (A1-A3, B1-B2, C1). Most dominant Type A1 alleles noted (46%), while B, B1 (10%).This first ever report molecular epidemiology variation Pvmsp-3β PCR/RFLP District Mardan showed a remarkable level studied circulating parasites area. results this will contribute future studies about structure vaccine development against malaria.