PCR priming from the restriction endonuclease site 3′extension
نویسندگان
چکیده
منابع مشابه
Characterization of a site-specific restriction endonuclease from Streptomyces aureofaciens.
A new type II sequence-specific restriction endonuclease, SauI, was isolated from Streptomyces aureofaciens IKA18/4. The purified enzyme was free of contaminating exonuclease and phosphatase activities. SauI cleaved lambda DNA at two sites, but did not cleave pBR322, simian virus 40, or phi X174 DNA. SauI recognized the septanucleotide sequence 5'-CCTNAGG-3' and cleaved at the position indicate...
متن کاملThe cleavage site of the restriction endonuclease Ava II.
We have determined that the type II restriction enzyme Ava II, isolated from Anabaena variabilis, recognizes and cuts the sequence (formula: see article). The eight Ava II sites of pBR322 have been mapped, as well as a unique site for Ava I.
متن کاملClassification of medically important clostridia using restriction endonuclease site differences of PCR-amplified 16S rDNA.
Restriction maps were constructed of enzymically amplified 16S rRNA genes (rDNA) isolated from eight Clostridium species. Using maximum parsimony, a dendrogram was constructed from these and published 16S rRNA sequence data. Two distinct clusters were identified: cluster I contained C. difficile, C. sordelli, and C. bifermentans, and showed 30 of 35 restriction sites in common; cluster II conta...
متن کاملThe cleavage site of the restriction endonuclease Ava II
We have determined that the type II restriction enzyme . Ava II , isolated from Anabaena variabilis, recognizes and cuts the sequence 5' G-1GTCC 3' 3 CCAG+G 5' The eight Ava II sites of pBR322 have been mapped, as well as a unique site for Ava
متن کاملMethylation analysis by restriction endonuclease digestion and real-time PCR.
DNA methylation is a highly characterized epigenetic modification of the human genome that is critical for normal cell processes, including tissue regulation, genetic expression development, genomic imprinting, X-chromosome inactivation, and DNA repair. DNA methylation in humans occurs almost exclusively at the C5 position of CpG dinucleotides in genomic promoter regions. Alterations in DNA met...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1993
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/21.20.4854