PCR for detection of Chlamydia trachomatis in endocervical, urethral, rectal, and pharyngeal swab samples obtained from patients attending an STD clinic.
نویسندگان
چکیده
منابع مشابه
PCR for detection of Chlamydia trachomatis in endocervical, urethral, rectal, and pharyngeal swab samples obtained from patients attending an STD clinic.
OBJECTIVE To investigate, by use of the Amplicor PCR in a routine setting, the recovery rate of Chlamydia trachomatis in ano-rectal and pharyngeal swab samples obtained from males and females attending an STD clinic in relation to sexual practices, symptoms, and signs. DESIGN Data regarding sexual practices, and symptoms and signs related to the rectum and pharynx, were obtained from 196 fema...
متن کاملCOMPARISON OF PCR BASED ASSAY AND CELL CULTURE IN DETECTION OF UROGENITA L CHLAMYDIA TRACHOMATIS INFECTION AND DETERMINATION OF THE INHIBITORY RATE O F SPECIMENS UNDER STUDY
An amplification polymerase chain reaction (PCR) test for the direct detection of Chlamydia trachomatis in urethral and endocervical swab specimens from symptomatic and asymptomatic women and men were compared to standard culture technique. During 6 months, 300 endocervical swab specimens from 205 asymptomatic women (64.4%) and 95 symptomatic women (31.6%), and 187 urethral swab specime...
متن کاملGenotyping of Chlamydia trachomatis from endocervical specimens in Shiraz, Iran
The objective of this study was to determine the prevalence and distribution of Chlamydia trachomatisgenotypes in Shiraz, Iran. Two hundred twelve cervical swab samples were collected from women attendingShahid Motahari Polyclinic in Shiraz, Iran. The endocervical specimens were screened for C. trachomatis byplasmid PCR. Genotyping was performed in C. trachomatis-positive samples by nested PCR ...
متن کاملEnzyme immunoassay for the detection of Chlamydia trachomatis antigen in urethral and endocervical swabs.
An enzyme immunoassay technique based on the direct detection of Chlamydia trachomatis antigen in urethral or cervical swabs was used for the rapid diagnosis of chlamydial genital infection. Urethral and cervical samples from 140 patients were tested in parallel by enzyme immunoassay and cell culture using iodine staining. The direct test had a sensitivity of 92.5% and specificity of 97.2% when...
متن کاملEvaluation of a new amplified enzyme immunoassay (EIA) for the detection of Chlamydia trachomatis in male urine, female endocervical swab, and patient obtained vaginal swab specimens.
AIMS To compare the performance of a new generation dual amplified enzyme immunoassay (EIA) with a molecular method for the diagnosis of Chlamydia trachomatis, using a range of urogenital samples, and to assess the reliability of testing self collected vaginal specimens compared with clinician collected vaginal specimens. METHODS Two population groups were tested. For the first population gro...
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ژورنال
عنوان ژورنال: Sexually Transmitted Infections
سال: 1997
ISSN: 1368-4973
DOI: 10.1136/sti.73.6.493