PCR-ELISA detection of Escherichia coli in milk
نویسندگان
چکیده
منابع مشابه
PCR-ELISA detection of Escherichia coli in milk.
AIMS The purpose of this study was to develop a reliable molecular procedure for the detection of Escherichia coli in milk. METHODS AND RESULTS Robust and expeditious DNA extraction and PCR techniques were evaluated using Enzyme-Linked Immunosorbent Assay (ELISA) detection of biotin-labelled amplicons to facilitate optimal detection of E. coli DNA. CONCLUSIONS It was found that 5 E. coli co...
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ژورنال
عنوان ژورنال: Letters in Applied Microbiology
سال: 2002
ISSN: 0266-8254,1365-2673
DOI: 10.1046/j.1472-765x.2002.01074.x