PCR driven DNA-DNA competitive hybridization: a new method for sensitive differential cloning
نویسندگان
چکیده
منابع مشابه
Development of a Sensitive Quantitative Competitive PCR Assay for Detection of Human Cytomegalovirus DNA
Accurate and rapid diagnosis of human cytomegalovirus (HCMV) disease in immunocompromised patients has remained as a challenge. Quantitative competitive PCR (QC-PCR) methods for detection of HCMV in these individuals have improved the positive and negative predictive values of PCR for diagnosis of HCMV disease. In this study we used QC-PCR assay, using a co-amplified DNA standard, to quantitate...
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Standardized procedures must be followed when characterizing, officially describing, and validly naming novel bacteria. For species descriptions, DNA-DNA hybridization still is needed for whole-genome comparisons between close relatives, but many established hybridization methods have drawbacks, such as requiring labeled or large amounts of DNA. We evaluated a new technique based on the spectro...
متن کاملdevelopment of a sensitive quantitative competitive pcr assay for detection of human cytomegalovirus dna
accurate and rapid diagnosis of human cytomegalovirus (hcmv) disease in immunocompromised patients has remained as a challenge. quantitative competitive pcr (qc-pcr) methods for detection of hcmv in these individuals have improved the positive and negative predictive values of pcr for diagnosis of hcmv disease. in this study we used qc-pcr assay, using a co-amplified dna standard, to quantitate...
متن کاملcolony pcr is a rapid and sensitive method for dna amplification in yeasts
background: yeast infections are increasing cause of morbidity and mortality in immunocompromised patients. in order to perform a dna-based diagnostic test, availability of a rapid and easy-to-perform dna extraction protocol is essential. in the present study we evaluated colony-pcr as the easiest way to amplification of target dna. methods: instead of using templates of purified genomic dna, w...
متن کاملA colorimetric method for DNA hybridization.
A general method has been developed which allows crosslinks to be produced between proteins and single-stranded DNA. Such single-stranded DNA protein complexes have been tested for blot hybridization using two colorimetrically detectable enzymes, namely peroxidase and alkaline phosphatase, as the protein moiety of the probe. After hybridization and incubation with a substrate solution sequences...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1991
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/19.17.4778