PCR-dipstick DNA Chromatography for detection of Carbapenemase Producing Carbapenem-Resistant Enterobacteriaceae
نویسندگان
چکیده
Carbapenemase producing carbapenem-resistant Enterobacteriaceae (CP-CRE) are an epidemiologically important emerging public health threat. Early and reliable detection of CP-CRE is for containment these pathogens. The aim this study was to evaluate observe the sensitivity polymerase chain reaction (PCR)-dipstick DNA chromatography (GeneFields CPE) carbapenemase genes (NDM, OXA-48, KPC, IMP) in comparison conventional PCR. In cross-sectional study, a total 145 carbapenem resistant isolates were subjected test by phenotypic mCIM genotypic- PCR PCR-dipstick Chromatography method. dipstick assay, after multiplex DNA-DNA hybridization-based system applied where amplified products can be easily interpreted observing blue line strip visually within 15 minutes without gel electrophoresis. All (100%) positive either NDM or OXA-48 whereas 142 (97.9%) detected positivity rate NDM, both among 73.8%, 51.7% 25.5% respectively. sensitivity, specificity assay 100.0%, 92.7% 98.6%, 97.2% This showed excellent agreement with CP-CRE. may robust diagnostic tool terms rapidity
منابع مشابه
The Problem of Carbapenemase-Producing-Carbapenem-Resistant-Enterobacteriaceae Detection.
The emergence and spread of carbapenemase-producing carbapenem-resistant Enterobacteriaceae (CP-CRE) are a significant clinical and public health concern. Reliable detection of CP-CRE is the first step in combating this problem. There are both phenotypic and molecular methods available for CP-CRE detection. There is no single detection method that is ideal for all situations.
متن کامل1800Phenotypic Definitions for Identifying Carbapenemase-Producing Carbapenem-resistant Enterobacteriaceae
1800. Phenotypic Definitions for Identifying Carbapenemase-Producing Carbapenem-resistant Enterobacteriaceae Nora Chea, MD; Sandra N. Bulens, MPH; Thiphasone Kongphet-Tran,; Valerie Albrecht,; Ruth Lynfield, MD; Kristin M Shaw, MPH, CIC; Marion Kainer, MBBS, MPH; Daniel Muleta,; Lucy Wilson, MD; Elisabeth Vaeth, MPH; Ghinwa Dumyati, MD; Cathleen Concannon, MPH; Erin C. Phipps, DVM; Karissa Culb...
متن کاملGuidance for control of infections with carbapenem-resistant or carbapenemase-producing Enterobacteriaceae in acute care facilities.
Infection with carbapenem-resistant Enterobacteriaceae (CRE) or carbapenemase-producing Enterobacteriaceae is emerging as an important challenge in health-care settings. Currently, carbapenem-resistant Klebsiella pneumoniae (CRKP) is the species of CRE most commonly encountered in the United States. CRKP is resistant to almost all available antimicrobial agents, and infections with CRKP have be...
متن کاملRapid Detection of Carbapenemase-producing Enterobacteriaceae
To rapidly identify carbapenemase producers in Enterobacteriaceae, we developed the Carba NP test. The test uses isolated bacterial colonies and is based on in vitro hydrolysis of a carbapenem, imipenem. It was 100% sensitive and specific compared with molecular-based techniques. This rapid (<2 hours), inexpensive technique may be implemented in any laboratory.
متن کاملRetrospective and prospective evaluation of the Carbapenem inactivation method for the detection of carbapenemase-producing Enterobacteriaceae
BACKGROUND There is an urgent need for accurate and rapid diagnostic tests to identify carbapenemase producing enterobacteria (CPE). Here, we have evaluated the Carbapenem Inactivation Method (CIM) test to detect CPEs from cultured colonies. METHODS A total of 256 enterobacterial isolates were used to evaluate the performance of the CIM in comparison to Carba NP test and molecular detection u...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: World Journal Of Advanced Research and Reviews
سال: 2023
ISSN: ['2581-9615']
DOI: https://doi.org/10.30574/wjarr.2023.18.1.0514