PCR-Dipstick Chromatography for Differential Detection of Carbapenemase Genes Directly in Stool Specimens
نویسندگان
چکیده
منابع مشابه
Detection of Opisthorchis viverrini in human stool specimens by PCR.
Opisthorchiasis is a liver fluke infection, found mainly in Southeast Asia (8) but increasingly in developed countries due to an influx of Asian immigrants (7). The diagnostic methods are based on the demonstration of eggs in stools, although there are still difficulties in distinguishing eggs from heterophyid and lecithodendriid parasites (2). We have successfully developed a PCR-based method ...
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OBJECTIVES To develop a single multiplex real-time PCR assay to detect six different genetic types of carbapenemases already identified in Enterobacteriaceae (KPC, GES, NDM, IMP, VIM and OXA-48). METHODS A total of 58 bacterial isolates were tested. Thirty were previously characterized as resistant to carbapenems and documented by PCR and sequencing analysis to carry the following genes: bla(...
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Carbapenem resistance among Enterobacteriaceae is an emerging problem worldwide. Klebsiella pneumoniae carbapenemase (bla(KPC)) enzymes are among the most common beta-lactamases described. In this study, we report the development and validation of a real-time PCR (q-PCR) assay for the detection of bla(KPC) genes using TaqMan chemistry. The q-PCR amplification of bla(KPC) DNA was linear over 7 l...
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BACKGROUND Efficacy of Helicobacter pylori stool antigen enzyme immunoassay (HpSA) and stool PCR was evaluated, before and after treatment, in a country with a high prevalence of H. pylori infection. METHODOLOGY A total of 52 patients with dyspeptic symptoms were included in the study. Antral biopsy was collected during pre- and post-therapy periods for rapid urease test (RUT) and PCR. Simila...
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An evaluation of the sensitivities of three DNA extraction methods, i.e., FTA filter paper, a QIAamp stool mini kit, and a conventional phenol-chloroform method, by using specimens with known concentrations of Enterocytozoon bieneusi spores was performed. FTA filter paper and the QIAamp stool mini kit were the most sensitive methods, which could detect E. bieneusi in specimens with a concentrat...
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ژورنال
عنوان ژورنال: Antimicrobial Agents and Chemotherapy
سال: 2017
ISSN: 0066-4804,1098-6596
DOI: 10.1128/aac.00067-17