Pattern of Indirect Immunofluorescence Assay Antinuclear Antibody in Pediatric Lupus Nephritis
نویسندگان
چکیده
منابع مشابه
An Antinuclear Antibody-Negative Patient With Lupus Nephritis
Systemic lupus erythematosus (SLE) is a typical autoimmune disease that's characterized by various autoantibodies to nuclear and cytoplasmic antigens. The presence of antinuclear antibodies (ANA) in serum is generally considered a decisive diagnostic sign of SLE. However, a small subset of SLE patients who had the typical clinical features of SLE was reported to show persistently negative ANA t...
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Background: Anti-nuclear antibodies (ANAs) are specific antibodies directed against a variety of nuclear antigens. Indirect immunofluorecence (ANA-IFA) and Enzyme Immunoassay (ANA-EIA) are commonly used methods for ANA detection. The positivity of ANA-IFA using HEp-2 cell substrate is 90-100% in systemic rheumatic diseases. On the other hand positivity of ANA-EIA is found to be much lower in di...
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BACKGROUND & OBJECTIVES Antinuclear antibodies (ANA) are serological hallmark of systemic lupus erythematosus (SLE). Conventionally, the test is carried out on human epithelial cells (HEp2) by indirect immunofluorescence (IIF) technique. Since culturing and maintaining HEp2 cells in the laboratory are labour intensive, in-house assays have given way to kits manufactured by commercial companies....
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Indirect immunofluorescence based on HEp-2 cell substrate is the most commonly used staining method for antinuclear autoantibodies associated with different types of autoimmune pathologies. The aim of this paper is to design an automatic system to identify the staining patterns based on block segmentation compared to the cell segmentation most used in previous research. Various feature descript...
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ژورنال
عنوان ژورنال: International Journal of Integrated Health Sciences
سال: 2020
ISSN: 2338-4506
DOI: 10.15850/ijihs.v8n1.1911