Pathogenicity of Vibrio parahaemolyticus causing acute hepatopancreatic necrosis disease (AHPND) in shrimp (Litopanaeus vannamei) in Serang, Banten, Indonesia

Abstract. Saputra A, Maftuch, Andayani S, Yanuhar U. 2023. Pathogenicity of Vibrio parahaemolyticus causing acute hepatopancreatic necrosis disease (AHPND) in shrimp (Litopanaeus vannamei) Serang, Banten, Indonesia. Biodiversitas 24: 2365-2373. Vannamei target EMS (Early Mortality Syndrome)/AHPND (Acute Hepotopancreatic Necrosis Disease) cases several Asian nations, including China, Vietnam, Malaysia, Thailand, India, and AHPND on farms have been reported Indonesian regions. The aim this study was to evaluate the histopathological pathogenicity different tissues exposed doses durations exposure. PCR amplification V. performed using Vp.flaE-79F (5?-GCA GCT GAT CAA AAC GTT GAG T-3?) Vp.flaE-934R (5?-GATACCTTGTTACGACTT -3?) primers for molecular identification bacteria isolated from exhibiting symptoms. positive band at 897 bp. Bacterial isolates with green colonies TCBS agar media were chosen other test. Challenge tests healthy conducted strain. challenge test demonstrated same clinical pathological symptoms as signs infected rivers, swimming into container’s corner changes carapace’s pale color empty midgut. results showed that challenged displayed necrosis, atrophy, granulomas, cell sloughing, had significantly considerable hemocyte infiltration led melanization. highest percentage (63.33%) damage recorded treatment a concentration 108 CFU mL-1. It also observed virulence strain varied concentrations. At concentrations mL-1 106 mL-1, caused 100% mortality 73 hour post-infection (p.i). lowest limit 104 48% p.i. causes main pathogen affecting vannamei