Partial purification and sugarcane bagasse induction of extracellular thermostable Amylase by Bacillus sp. under submerged fermentation
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چکیده
منابع مشابه
PRODUCTION AND CHARACTERIZATION OF THERMOPHILIC CARBOXYMETHYL CELLULASE SYNTHESIZED BY Bacillus sp. GROWING ON SUGARCANE BAGASSE IN SUBMERGED FERMENTATION
The production and characterization of cellulase from thermophilic strain Bacillus sp. C1AC5507 was studied. For enzyme production, sugarcane bagasse was used as carbon source. The produced carboxymethyl cellulase (CMCase) had a molecular weight around 55 kDa and its activity varied between 0.14 and 0.37 IU mL in conditions predicted by Response Surface Methodology. The optimum temperature and ...
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This study investigates the production of crude Ca-independent and low pH active α-amylase by Bacillussp. KR-8104 in submerged fermentation (SmF) and solid-state fermentation (SSF) systems. Differentparameters were evaluated in each system using “one factor at a time” approach to improve the production ofenzyme. The results showed that in the SmF the maximum enzyme production ...
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In most of the raw materials of plant origin used in animal feed, a portion of the phosphorus is stored as phytic acid or phytate. Phytate is the main storage form of phosphorus in vegetables but is not readily assimilated into food at low concentrations of the enzyme phytase. In addition to making phosphorous unavailable, phytate binds divalent cations such as calcium, copper, magnesium, iron,...
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The effect of several nutritional and environmental parameters on Penicillium purpurogenum growth and sacharogenic amylase production was analyzed. High enzyme levels (68.2 U mg(-1)) were obtained with Khanna medium at initial pH 6.0, incubated at 30°C for 144 hours. The optimum pH and temperature activities were 5.0 and 65°C, respectively. The enzyme presented a half-life (t50) of 60 min, at 6...
متن کاملOptimization, production and partial purification of extracellular α- amylase from Bacillus sp. marini
The aim of the current study was to isolate amylase producing bacteria from the soil samples collected from marine environment of Andaman and Nicobar Islands, India. The isolation was performed by serial dilution and plating method. Total 35 bacteria were isolated from the collected soil samples. All isolates were screened for amylolytic activity by starch agar plate method, among 35 bacterial ...
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ژورنال
عنوان ژورنال: Canadian Journal of Biotechnology
سال: 2017
ISSN: 2560-8304
DOI: 10.24870/cjb.2017-a142