Parthenogenetic Activation of Rhesus Monkey Oocytes and Reconstructed Embryos1
نویسندگان
چکیده
منابع مشابه
Parthenogenetic activation of rhesus monkey oocytes and reconstructed embryos.
This study determines the efficiency of sequential calcium treatments (electroporation or ionomycin) combined with protein synthesis (cycloheximide) or phosphorylation inhibitors (6-dimethylaminopurine) or the specific maturation promoting factor (MPF) inhibitor, roscovitine, in inducing artificial activation and development of rhesus macaque parthenotes or nuclear transfer embryos. Exposure of...
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The aim of the study was to compare the parthenogenetic activation and in vitro fertilization (IVF) of in vitro matured caprine oocytes. A total of 881 cumulus-oocyte complexes (COC’s) were collected from 243 ovaries. Oocytes were matured in TCM-199 medium containing eCG (20 IU/ml), hCG (20 IUμg/ml), oestradiol-17β (1 μg/ml), BSA embryo tested (3 mg/ml) supplemented with 10% fetal bovine serum ...
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Myocardial necrosis can be result from a number of causes including nutritional deficiencies, chemical and plant toxins, ischemia and metabolic disorder. The outcome of myocardial necrosis varies depending on the extent of the damage (Donald 2001, Jubb 1993, Radostits 1994, Vanvaleet 1986). Myocardial infarction without demonstrable of atherosclerosis were reported in a rhesus macaque (Gonder 1...
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Purpose: Parthenogenetic activation is among the crucial steps determining successful development of mammalian cloned embryos. This study, therefore, was conducted to evaluate the efficiency of a novel combined electrical-chemical artificial activation method for bovine cloning. Materials and Methods: In vitro matured bovine oocytes than were initially exposed to electrical pulse, used for cel...
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These experiments were carried out to optimize the parameters of electrical activation, methods of parthenogenetic activation and embryo culture in vitro and meanwhile to isolate embryonic stem cells-like (ESCs) derived from porcine parthenogenetic blastocysts (pPBs). These results showed that, as the electric field strength increased from 1.0 to 2.7 kV/cm, the cleavage rate of parthenogenetic ...
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ژورنال
عنوان ژورنال: Biology of Reproduction
سال: 2001
ISSN: 0006-3363,1529-7268
DOI: 10.1095/biolreprod65.1.253