Parthenogenetic Activation of In Vitro Matured Bovine Oocytes
نویسندگان
چکیده
منابع مشابه
A comparative study of parthenogenetic activation and in vitro fertilization of in vitro matured caprine oocytes
The aim of the study was to compare the parthenogenetic activation and in vitro fertilization (IVF) of in vitro matured caprine oocytes. A total of 881 cumulus-oocyte complexes (COC’s) were collected from 243 ovaries. Oocytes were matured in TCM-199 medium containing eCG (20 IU/ml), hCG (20 IUμg/ml), oestradiol-17β (1 μg/ml), BSA embryo tested (3 mg/ml) supplemented with 10% fetal bovine serum ...
متن کاملA comparative study of parthenogenetic activation and in vitro fertilization of in vitro matured bovine oocytes
The objective of this study was to determine the effects of 3 chemical agents used sequentially and electrical stimuli on parthenogenetic activation of in vitro matured bovine oocytes and comparison with a standard IVF protocol for embryo developmental rates. For this purpose, oocytes were matured in tissue culture medium-199 (TCM-199) at 39 °C and 5% of CO2 in humidified air. For IVF, matured ...
متن کاملOptimized combined electrical-chemical parthenogenetic activation for in vitro matured bovine oocytes.
Sperm-mediated oocyte activation is a complex procedure, both in steps and duration, not yet been completely mimicked during in vitro studies, e.g., parthenogenesis or somatic cell nuclear transfer. Furthermore, parthenogenetic studies have been recognized as a suitable model for studying activation efficiency for nuclear transfer cloning. This study, therefore, was conducted to develop an opti...
متن کاملa comparative study of parthenogenetic activation and in vitro fertilization of in vitro matured caprine oocytes
the aim of the study was to compare the parthenogenetic activation and in vitro fertilization (ivf) of in vitro matured caprine oocytes. a total of 881 cumulus-oocyte complexes (coc’s) were collected from 243 ovaries. oocytes were matured in tcm-199 medium containing ecg (20 iu/ml), hcg (20 iuμg/ml), oestradiol-17β (1 μg/ml), bsa embryo tested (3 mg/ml) supplemented with 10% fetal bovine serum ...
متن کاملP-118: Effect of Vitrification on Developmental Competence of Parthenogenetic Activation in In Vitro Matured Ovine Oocytes
Background: Cryopreservation of in vitro matured oocytes is a useful technique because the oocytes can be used for some assisted reproductive technologies. On the other hand, the cryopreservation of oocytes is an open problem as a result of their structural sensitivity to the freezing process. The purpose of this study was to evaluate the effect of vitrification on in vitro development of vitri...
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ژورنال
عنوان ژورنال: Journal of Dairy Science
سال: 1993
ISSN: 0022-0302
DOI: 10.3168/jds.s0022-0302(93)77362-2