Park-Williams Number 8 Strain of Corynebacterium diphtheriae
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چکیده
منابع مشابه
Park-Williams number 8 strain of Corynebacterium diphtheriae.
Five clones of the Park-Williams number 8 strain of Corynebacterium diphtheriae, previously maintained in separate laboratories, were examined for their colonial and biochemical properties, for the restriction and modification system which operates to obscure their lysogeny, and for their capacity to produce large amounts of toxin under ordinary laboratory conditions. The phenotypes of their ph...
متن کاملExpression and purification of the immunogenically active fragment B of the Park Williams 8 Corynebacterium diphtheriae strain toxin.
The construction of a hexahistidine-tagged version of the B fragment of diphtheria toxin (DTB) represents an important step in the study of the biological properties of DTB because it will permit the production of pure recombinant DTB (rDTB) in less time and with higher yields than currently available. In the present study, the genomic DNA of the Corynebacterium diphtheriae Park Williams 8 (PW8...
متن کاملRestriction endonuclease map of corynebacteriophage omega ctox+ isolated from the Park-Williams no. 8 strain of Corynebacterium diphtheriae.
The toxigenic corynebacteriophage omega tox+ was isolated from the hypertoxigenic Park-Williams no. 8 (PW8) strain of Corynebacterium diphtheriae and compared with the toxigenic corynebacteriophage beta tox+. The physical size and host range of both phages were found to be identical. An endonuclease restriction map of omega tox+ was constructed, and the locations of the cohesive ends (cos), pha...
متن کاملThe cytology of a strain of Corynebacterium diphtheriae.
Organism studied. Corynebacteriunm diphtheriae, type mnitis, strain A9255. Growth media included M. and E. (AMorton and Engley, 1945) broth and agar, with and without enrichment by whole blood, plasma or the lysate from human blood cells subjected to freezing and thawing. Culture was usually at 37 C; occasionally, at room temperature. Stained preparations. Cells in broth culture were placed on ...
متن کاملIsolation of a cured strain from Corynebacterium diphtheriae PW8.
A nonlysogenic, non-toxinogenic strain was isolated from the PW8 strain of C. diphtheriae by two-step ultraviolet induction. This strain was lysed by phages derived from the PW8 strain but not by beta phages from the C7(beta) strain. When this cured strain was lysogenized with phages from the PW8 strain, toxin production by the resulting lysogens was about a half or a quarter of that of the par...
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 1971
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.105.1.77-85.1971