PapG subtype-specific binding characteristics of Escherichia coli towards globo-series glycosphingolipids of human kidney and bladder uroepithelial cells
نویسندگان
چکیده
منابع مشابه
Adhesion of Escherichia coli to human uroepithelial cells in vitro.
Optimal conditions for in vitro adherence of Escherichia coli to uroepithelial cells, previously shown to more efficient for strains causing acute symptomatic than that for strains causing "asymptomatic" urinary tract infections, were investigated. Uroepithelial cells from fresh morning urine of healthy individuals and E. coli bacteria from patients with various forms of urinary tract infeciton...
متن کاملSynthesis of Complex Glycosphingolipids of the Globo Series
Versatile strategies were designed for the synthesis of biologically important glycosphingolipids of the globo series, as illustrated for the synthesis of the hexasaccharide of the human breast cancer antigen (Globo H, MBr1) and of the stage-specific embryonic antigen 4 (SSEA-4, Sialylgalactosylgloboside).
متن کاملDetection of intracellular uropathogenic Escherichia coli during an in vitro infection of bladder uroepithelial cells
Introduction: Urinary tract infection (UTI) is the most common bacterial infection in women. Uropathogenic Escherichia coli (UPEC) cause ~80% of all community-acquired UTIs. Although UTIs are traditionally classified as acute infections, the recurrence-frequency is relatively high. Recurrent UTIs are conventionally thought of as reinfections, but recent studies have shown that UPEC-strains use ...
متن کاملStructural basis of the preferential binding for globo-series glycosphingolipids displayed by Pseudomonas aeruginosa lectin I.
The opportunistic pathogen Pseudomonas aeruginosa contains several carbohydrate-binding proteins, among which is the P. aeruginosa lectin I (PA-IL), which displays affinity for alpha-galactosylated glycans. Glycan arrays were screened and demonstrated stronger binding of PA-IL toward alphaGal1-4betaGal-terminating structures and weaker binding to alphaGal1-3betaGal ones in order to determine wh...
متن کاملHuman Bladder Uroepithelial Cells Synergize with Monocytes to Promote IL-10 Synthesis and Other Cytokine Responses to Uropathogenic Escherichia coli
Urinary tract infections are a major source of morbidity for women and the elderly, with Uropathogenic Escherichia coli (UPEC) being the most prevalent causative pathogen. Studies in recent years have defined a key anti-inflammatory role for Interleukin-10 (IL-10) in urinary tract infection mediated by UPEC and other uropathogens. We investigated the nature of the IL-10-producing interactions b...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Glycobiology
سال: 2019
ISSN: 1460-2423
DOI: 10.1093/glycob/cwz059