Paper Chromatography of Tannins

Study of phenylketonuria by paper chromatography

Paper Chromatography

In this laboratory exercise we will separate and identify the cations in an aqueous mixture that possibly contains Fe 3+ , Ni 2+ or Cu 2+ salts. The separation will be achieved using Paper Chromatography. Identification of the cations will be affected by simultaneously running separate solutions, each containing a single salt of these ions, through the chromatographic system and comparing the r...

Improved Characterization of Sorghum Tannins Using Size-Exclusion Chromatography

Sorghum (Sorghum bicolor L. Moench) is a native grass species of the arid and semi-arid regions of Africa (Kimber 2000). Sorghum is a genetically diverse crop and contains some genotypes that have a pigmented testa and therefore contain tannins (Rooney et al 1980; Rooney and Miller 1982). Such lines have the dominant B1_B2_ genes and are the only sorghum types with tannins (Blakeley et al 1979)...

Paper Chromatography of Steroids

In Paper I of this series (1) a method was described for the paper chromatographic analysis of certain ketonic steroids as hydrazones formed with Girard’s Reagent T. When that technique was applied to the adrenal cortical hormones, however, certain deficiencies appeared. (a) The RI values of the individual steroid-Reagent T hydrazones were not sufficiently different to permit identification. (b...

Paper chromatography of amines.