Paper Chromatography of Organic Acids

Separation of Amino Acids by Paper Chromatography

Chromatography is a common technique for separating chemical substances. The prefix “chroma,” which suggests “color,” comes from the fact that some of the earliest applications of chromatography were to separate components of the green pigment, chlorophyll. You may have already used this method to separate the colored components in ink. In this experiment you will use chromatography to separate...

Study of phenylketonuria by paper chromatography

Paper chromatography of free amino acids in human blood serum.

BOTJLANGER and BISERrE (1) used a solution of acid acetone for the extraction of amino acids in biologic material. The separation of amino acids on buffered papers described by Ryb#{225}r, Touek, and Hais, (2) and Hais and Hordovs/q (3) used the combination of tertiary butanolborate buffer system (pH 8.5) for separation of amino acids on paper. In our laboratories we have developed a chromatogr...

Determination of monoamino monocarboxylic acids by quantitative paper chromatography.

Many methods for chromatographic separation of amino acids are now available, but their subsequent determination, generally employing ninhydrin, often leaves much to be desired. It was felt, therefore, that there was a need for a simple method of analysis based on the convenient paper technique and employing some means of determination less fickle than ninhydrin colorimetry. The method now pres...

Separation and estimation of organic acids on paper chromatograms.