Paper Chromatography of Dye Intermediates

Study of phenylketonuria by paper chromatography

Triazine-dye affinity; chromatography.

functional resemblance of column groups to carbamoyl phosphate. The enzyme binds its two substrates, carbamoyl phosphate and L-aspartate, in an obligatory order, with carbamoyl phosphate leading (Grayson et al., 1979). If column groups are sufficiently similar to carbamoyl phosphate, the enzyme should be adsorbed more tightly in the presence of L-aspartate, owing to the ‘locking-on’ effect (O’C...

Paper Chromatography

In this laboratory exercise we will separate and identify the cations in an aqueous mixture that possibly contains Fe 3+ , Ni 2+ or Cu 2+ salts. The separation will be achieved using Paper Chromatography. Identification of the cations will be affected by simultaneously running separate solutions, each containing a single salt of these ions, through the chromatographic system and comparing the r...

Paper Chromatography of Steroids

In Paper I of this series (1) a method was described for the paper chromatographic analysis of certain ketonic steroids as hydrazones formed with Girard’s Reagent T. When that technique was applied to the adrenal cortical hormones, however, certain deficiencies appeared. (a) The RI values of the individual steroid-Reagent T hydrazones were not sufficiently different to permit identification. (b...

Paper chromatography of amines.