Paper chromatography of cholesterol and desmosterol after bromination

Resolution of desmosterol, cholesterol, and other sterol intermediates by reverse-phase high-pressure liquid chromatography.

A highly efficient technique has been developed for the resolution of several sterols that are intermediates in the biosynthesis of cholesterol and that differ only by one carbon-carbon double bond or by one methyl group. The technique described utilizes reverse-phase high-pressue liquid chromatography on a micronBondapak-C18 column with acetonitrile as eluting solvent. This procedure is capabl...

Protein Bands Detection by Nanoparticles after Paper Chromatography

Paper chromatography is an analytical technique for separating and identifying mixtures of materials. It is a useful technique since it is relatively quick, and requires small quantities of the material. By emergence of nanotechnology, paper chromatography has found many applications in biology and biotechnology. In this study, we employed gold and silver nanoparticles to detect protein bands a...

The Estimation of Desmosterol and Cholesterol in Plasma.

Desmosterol (24-dehydrocholesterol) is the immediate precursor of cholesterol in the biosynthesis of the latter compound. The structures of these two sterols are very similar, and desmosterol interferes with many of the colorimetric methods used for estimating plasma cholesterol levels. Normally, desmosterol is not present in plasma in significant amounts, but in patients treated with triparano...

Study of phenylketonuria by paper chromatography

protein bands detection by nanoparticles after paper chromatography

paper chromatography is an analytical technique for separating and identifying mixtures of materials. it is a useful technique since it is relatively quick, and requires small quantities of the material. by emergence of nanotechnology, paper chromatography has found many applications in biology and biotechnology. in this study, we employed gold and silver nanoparticles to detect protein bands a...