Paper Chromatographic Separation of Thiamine Phosphates.

A thin-layer-chromatographic method for the separation of sugar phosphates.

nutrient-agar plate was replica-plated on to nitrogen-limiting, 1 % lactose minimal medium. Several replicas of each plate were made and were incubated at 30°C for 3 days to guarantee maximal glycogen accumulation. The temperature was then increased to 40°C and an adequate nitrogen source added. Being lactose temperature-sensitive, strain LM 136 is incapable of utilizing lactose at 40°C and the...

Chromatographic separation of glycated nucleotides.

A high-performance liquid chromatographic method for the separation of nucleotide glycation reaction product(s) has been developed. The product(s) arising during in vitro glycation of a nucleotide (AMP, GMP or CMP) with a reducing sugar (ribose or glucose) were clearly resolved from the non-glycated constituents of the reaction mixture. The peak(s) of the glycated product(s) increased when an a...

Chromatographic Separation of the Diastereoisomers

Reduction of folic acid to the tetrahydro level introduces a second asymmetrical center into the molecule at carbon atom 6.1 The resulting tetrahydrofolate, and subsequent derivatives, prepared chemically consist of dl mixtures that are only 50% active in biological reactions. The need for fully reactive isomers of reduced folates led to the enzymatic synthesis of tetrahydrofolate from 7, %dihy...

[Paper chromatographic analysis of plasmalogens].

Paper-chromatographic Separation of [3h]borohydride- Reduced Hexoses for Quantitative Determination of Polysaccharide Composition

An improved procedure for determination of composition of heteropolysaccharides by radiochromatography of sodium [3H]borohydride-reduced hydrolyzates has been developed. The method is accurate and sensitive, is less cumbersome and more reliable than similar procedures currently in use, and permits, for the first time, compositional analysis of hydrolyzates containing both hexoses and uronic aci...