P10.05.B Platelet-derived growth factor signalling pathways in patient-derived glioblastoma cells

Abstract Background Platelet-derived growth factor (PDGF) signalling is essential in the development and maintenance of neurovasculature. Of particular importance PDGFRβ for recruitment mural cell type pericytes, which occupy an important position coordinating blood-brain barrier functions. The PDGF pathway also implicated glioblastoma, where overexpression PDGFRα a signature proneural subtype highly malignant invasive tumour. expression both β by tumour cells have been tumorigenesis progression. Therefore, we sought to study receptor primary human-derived glioblastoma gain better understanding mechanisms driven these receptors. Material Methods Primary human epilepsy pericytes were isolated from surgical resections obtained consenting patients at Auckland City Hospital. pathways investigated through treatment with exogenous ligands. Pathway activation was quantified using immunocytochemistry, cytokine XL Proteome Profiler cytometric bead arrays. Results PDGF-BB PDGF-DD led pericyte cultures, mediated mitogen-activated protein kinase (MAPK) phosphoinositide 3-kinase (PI3K)/Akt cascades, ultimately increasing proliferation secretion. PDGF-AA resulted transient MAPK activation, but not followed increased pericytes. In contrast, although PDGF-AA, -BB -DD induced internalisation (and internalisation) GBM cells, this did result or PI3K/Akt proliferation. Conclusion Despite expression, contrast surprisingly displayed lack responses ligand stimulation either PDGFRβ. This warrants further investigation into behind understand biology.