P-346 Migration-associated microRNAs are dysregulated in endometriosis: potential diagnostic biomarkers

Abstract Study question What is the role of dysregulated endometrial microRNAs (miRNAs) in development endometriosis? Summary answer Dysregulated miRNAs endometrium women with endometriosis can also be found endometriomas and may affect migratory ability endometriotic cells. known already The molecular mechanisms underlying pathogenesis are poorly understood. One hypotheses that changes cell properties observed lesions could initiated endometrium. Dysregulation has been reported previous microarray-based studies. However, little overlap seen between published miRNA expression data. Moreover, potential whether these present endometrioma largely unknown. design, size, duration In this experimental case-control study gene proliferative phase was compared 15 laparoscopically confirmed (stage III–IV) 17 age-matched controls who were to free endometriosis. Selected further paired proliferative-phase from a new cohort studied vitro understand their effect on migration. Participants/materials, setting, methods Samples collected during laparoscopic operations performed at Tartu University Hospital, Estonia. detected small RNA sequencing differential analysis. Target genes biological pathways predicted disease. for using qRT-PCR in-vitro proliferation migration transwell-migration assay after mimic transfection 12Z line. Main results chance total, we identified 9 upregulated 5 downregulated (-2 <fold change > 2, FDR < 0.05) controls. In-silico analyses showed target significantly enriched migration-related KEGG such as adherens junctions, focal adhesion, MAPK-, PI3-AKT-, TGF-beta signaling. most down-regulated miRNA, miR-193b-5p, up-regulated miR-374b-5p, selected characterization. Validation significant up-regulation both (Fold 0.05). Since it altered involved our associated pathways, explored if miR-193b-5p affects capacity A 2-fold decrease (p-value 0.0021) transfection. No demonstrated. Limitations, reasons caution Although findings in-silico suggest link migration, studies primary cells in-vivo animal models needed reveal specific regulate explain functional context Wider implications This gives insight into endometriosis, understood disease, by demonstrating potentially linked changed ability. Furthermore, evaluated diagnostic biomarkers larger Trial registration number Not applicable