P-202 Increased expression of Noggin in pancreatic cancer is associated with patients' poor survival

Noggin is a secreted antagonist of bone morphogenetic proteins (BMPs) and involved in the regulation developmental, homeostatic neoplastic biological processes. Whilst BMPs have been implicated many forms cancer, effect noggin on pancreatic cancer remains still poorly understood. Present study aims to examine role played by cancer. Expression was analysed cohort comprising tumours (n=149) paired adjacent issues (n=145) which were collected at Beijing Cancer Hospital with informed consent from patients. Protocols procedure tissue collection processing reviewed approved Ethic Committee. expression also evaluated 36 ductal adenocarcinoma matching normal samples (GSE15471). The t-test, Mann-Whitney test One-way ANOVA for its implication tumour grade, local invasion, lymph node distant metastases. Survival analysis patients using Kaplan-Meier Genome Atlas (TCGA) cohort. Correlation between epithelial mesenchymal transition (EMT) markers Spearman test. Knockdown performed two cell lines (PANC-1 MiaPaCa2) lentiviral shRNAs comparison scramble shRNA as control. Impact proliferation determined vitro both 2D 3D culture models. shown be higher tissues compared mRNA level significantly clinical well GSE15471 dataset. dramatically elevated T3 versus T1 staging significantly. Increased presents correlation poorer overall survival relapse free survival. Patients had median being 15.33 months, p < 0.001 23.17 months those lower tumours. knockdown resulted an increase growth spheroids PANC-1 cells but little impact MiaPaCa2 cells. Data analyses showed that positively correlated EMT biomarkers such slug, twist1 vimentin certain MMPs. up regulated High associated However, cellular molecular machinery underlying yet further investigated particular potential invasive trait.