P-121 Identification of differentially expressed genes common to the developmental potential, maternal age, and morphology in human blastocysts

Abstract Study question Is it possible to apply RNA-sequencing assisted reproductive technology (ART) predict blastocyst quality? Summary answer We identified 14 commonly differentially expressed genes by grouping blastocysts according three distinct parameters such as developmental potential, maternal age, and Gardner score What is known already In ART, while the selection of a suitable embryo for transfer critical successful pregnancy, neither preimplantation genetic testing nor morphological chronological evaluation can fully guarantee pregnancy. Recently, transcriptional events in early human embryonic development have been analyzed using (RNA-seq) researchers are attempting this information ART. reported that 96 (DEGs) were RNA-seq each inner cell mass (ICM) trophectoderm (TE) classified potential which correlates with pregnancy rate at ESHRE 37th annual meeting. design, size, duration After retrospectively analyzing 1,890 cases undergoing freeze-thaw from March 2018 December 2020 examine correlation between rate, total 13 cryopreserved February 2011 September 2018, then scheduled disposal consented, subjected identify associated expectation. data examined whether common DEGs could be found when age score, respectively. Participants/materials, setting, methods Blastocysts donated infertile couples c-IVF or ICSI cycles Yamashita Shonan Yume Clinic informed consent under ethical approval. TE cells ICM collected DEGs. addition, regrouped find (q-value < 0.01) R package “DESeq2” (version 1.32.0). Main results role chance When obtained expectation re-grouped re-analyzed, we 7 17 down- up-regulated elder group, respectively, ICM. TE, 2 12 On other hand, (<BB versus <BB), 26 29 poor 64 20 A comparison these genes. By one gene (UCHL1) was TE. (CMTM7) down-regulated (LINC00458, DYNLRB1, UBL4A) ICM, 5 (LRIG3, SLC28A3, CXADR, LOC727896, ATP1B1) (UCHL1, BZW2, NABP1, HEXIM1, PLEKHA6) Limitations, reasons caution Although established an expected concerning degree retrospective clinical outcomes used surrogate marker assigning biopsied different analysis groups, remains unknown expression profiles accurately reflect outcomes. Wider implications findings UCHL1 increased lower higher score. Since has essential mice, our suggest may also quality humans. Trial registration number not applicable