Oxygen concentration modulates neonatal mouse cardiomyocyte’s fate in vitro
نویسندگان
چکیده
منابع مشابه
Isolation and culture of neonatal mouse cardiomyocytes.
Cultured neonatal cardiomyocytes have long been used to study myofibrillogenesis and myofibrillar functions. Cultured cardiomyocytes allow for easy investigation and manipulation of biochemical pathways, and their effect on the biomechanical properties of spontaneously beating cardiomyocytes. The following 2-day protocol describes the isolation and culture of neonatal mouse cardiomyocytes. We s...
متن کاملAKIP1 Expression Modulates Mitochondrial Function in Rat Neonatal Cardiomyocytes
A kinase interacting protein 1 (AKIP1) is a molecular regulator of protein kinase A and nuclear factor kappa B signalling. Recent evidence suggests AKIP1 is increased in response to cardiac stress, modulates acute ischemic stress response, and is localized to mitochondria in cardiomyocytes. The mitochondrial function of AKIP1 is, however, still elusive. Here, we investigated the mitochondrial f...
متن کاملTrypanosoma cruzi modulates the expression of Rabs and alters the endocytosis in mouse cardiomyocytes in vitro.
Chagas disease is an incurable illness caused by the protozoan Trypanosoma cruzi. Cardiomyocytes represent important targets for the parasite infection and alterations in their physiology were reported. Because endocytosis is involved in different cellular events and guanosine triphosphatase (GTPase) Rab proteins play important roles in various aspects of the membrane traffic, our aim was to ch...
متن کاملRelaxin promotes growth and maturation of mouse neonatal cardiomyocytes in vitro: clues for cardiac regeneration
The demonstration that the adult heart contains myocardial progenitor cells which can be recruited in an attempt to replace the injured myocardium has sparkled interest towards novel molecules capable of improving the differentiation of these cells. In this context, the peptide hormone relaxin (RLX), recently validated as a cardiovascular hormone, is a promising candidate. This study was design...
متن کاملkinetic properties of lactate dehydrogenase extraeted from mouse embryonic stem cell-derived cardiomyocytes and mouse neonatal cardiomyocytes: a comparative study
introduction: lactate dehydrogenase is one of the cardiac enzyme markers, studied in vivo. in the present study, activity and kinetic properties of enzyme extracted from cardiomycytes derived from mouse embyronic stem cell in vitro were compared with enzyme extracted mouse neonatal from cardiomyocytes in vivo. material and methods: mouse embryonic stem cells were cultured and differentiated and...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Archives of Cardiovascular Diseases Supplements
سال: 2017
ISSN: 1878-6480
DOI: 10.1016/s1878-6480(17)30444-5