OxLDL-Induced Foam Cell Formation Inhibitory Activity of Pepsin Hydrolysate of Ark Shell (Scapharca subcrenata (Lischke, 1869)) in RAW264.7 Macrophages

Inhibitory effect of ark shell (Scapharca subcrenata (Lischke, 1869)) proteolytic hydrolysates (ASHs) on oxidized low-density lipoprotein (oxLDL)-induced macrophage foam cell formation was investigated. Two types ASHs were prepared by Alcalase® and pepsin, ASAH (ark shell-Alcalase® hydrolysates), ASPH shell-pepsin hydrolysate). Oil Red O staining results showed that suppressed lipid accumulation more than in oxLDL-induced RAW264.7 macrophages. reduced the levels total cholesterol, cholesterol ester, free oxLDL-treated It found increased efflux decreased influx rate. In this regard, protein expressions CD36 scavenger receptor class A1 (SR-A1) for ATP-binding cassette transporter G1 (ABCA1 ABCG1) treatment resulted an increase ABCA1 ABCG1 expression but downregulated SR-A1 expression. Furthermore, production proinflammatory cytokines, including tumor necrosis factor-α interleukin-6 -1β, through regulating nuclear factor-kappa B (NF-κB) Taken together, our data indicate might be a useful ingredient functional foods ameliorating atherosclerosis preventing formation.