Oxidative denitrification of Nω-hydroxy-l-arginine by the superoxide radical anion
نویسندگان
چکیده
منابع مشابه
Oxidative denitrification of Nω-hydroxy-L-arginine by the superoxide radical anion
The superoxide radical anion (O # −d) produced during the catalytic activity of nitric oxide synthase (NOS) and cytochrome P-450 has been implicated in the oxidative denitrification of hydroxyguanidines ("C ̄NOH). The reactivity of the radiolytically generated O # −d radical with N-hydroxy--arginine (NHA) is pH dependent and appears to parallel the prototropic equilibrium of the hydroxyguanidin...
متن کاملThe Inhibition of Arginase by Nω-Hydroxy-l-Arginine Controls the Growth of Leishmania Inside Macrophages
Polyamine synthesis from l-ornithine is essential for Leishmania growth. We have investigated the dependence of Leishmania infection on arginase, which generates l-ornithine, in macrophages from BALB/c, C57BL/6, and nitric oxide synthase II (NOS II)-deficient mouse strains. We have found that N(omega)-hydroxy-l-arginine (LOHA), a physiological inhibitor of arginase, controls cellular infection ...
متن کاملSynthesis of nitric oxide from L-arginine by neutrophils. Release and interaction with superoxide anion.
Stimulated rat peritoneal neutrophils release a platelet inhibitory factor with the pharmacological properties of NO. This release is inhibited by NG-monomethyl-L-arginine and L-canavanine, indicating that it occurs through a mechanism similar to that in vascular endothelial cells and macrophages. As the degree of stimulation increases, the factor released is progressively inactivated by concom...
متن کاملSuperoxide anion radical (O2) generation by adriamycin and anthraquinone analogues.
The reduction of ferricytochrome c with adriamycin in phospate buffered saline (pH 7.4) and alkaline solution was obsereved spectrophotometrically. The reduction increased gradually with time of incubation, and decreased with the addition of superoxide dismutase. The semiquinone radical derived from adriamycin was detected by electron spin resonance spectrometer. Superoxide production by analog...
متن کاملEnzymatic and Cryoreduction EPR Studies of the Hydroxylation of Methylated Nω-Hydroxy-l-arginine Analogues by Nitric Oxide Synthase from Geobacillus stearothermophilus
Nitric oxide synthase (NOS) catalyzes the conversion of L-arginine to L-citrulline and NO in a two-step process involving the intermediate N(ω)-hydroxy-L-arginine (NHA). It was shown that Cpd I is the oxygenating species for L-arginine; the hydroperoxo ferric intermediate is the reactive intermediate with NHA. Methylation of the N(ω)-OH and N(ω)-H of NHA significantly inhibits the conversion of...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1996
ISSN: 0264-6021,1470-8728
DOI: 10.1042/bj3170017