Overexpression and optimization of glutamate decarboxylase in L actobacillus plantarum Taj‐Apis 362 for high gamma‐aminobutyric acid production

Overexpression and optimization of glutamate decarboxylase in Lactobacillus plantarum Taj-Apis362 for high gamma-aminobutyric acid production

Gamma-aminobutyric acid (GABA) is an important bioactive compound biosynthesized by microorganisms through decarboxylation of glutamate by glutamate decarboxylase (GAD). In this study, a full-length GAD gene was obtained by cloning the template deoxyribonucleic acid to pTZ57R/T vector. The open reading frame of the GAD gene showed the cloned gene was composed of 1410 nucleotides and encoded a 4...

cloning of glutamate decarboxylase and production of γ-aminobutyric acid from lactobacillus brevis

Overexpression, purification, crystallization and preliminary structural studies of p-coumaric acid decarboxylase from Lactobacillus plantarum.

The substrate-inducible p-coumaric acid decarboxylase (PDC) from Lactobacillus plantarum has been overexpressed in Escherichia coli, purified and confirmed to possess decarboxylase activity. The recombinant His(6)-tagged enzyme was crystallized using the hanging-drop vapour-diffusion method from a solution containing 20%(w/v) PEG 4000, 12%(w/v) 2-propanol, 0.2 M sodium acetate, 0.1 M Tris-HCl p...

Accumulation of γ-aminobutyric acid and transcription of glutamate decarboxylase in Brassica juncea (L.) Czern

Mustard leaf (Brassica juncea (L.) Czern.) using as material of a traditional fermented vegetable food (Kimchi) in Korea, is one of the important vegetables. Two cultivars of mustard (red and green) were grown in the experimental farm of Chungnam National University and allowed them to grow until flowering stage. In this study, the variability of γ-aminobutyric acid (GABA) content and mRNA tran...

Overexpression of cysteine sulfinic acid decarboxylase stimulated by hepatocarcinogenesis results in autoantibody production in rats.

We developed a novel and efficient cDNA subtraction method to isolate rat hepatocellular carcinoma (HCC)-related genes. cDNAs from Solt-Farber procedure-driven HCCs were synthesized on Latex beads. The subtraction was accomplished by a simple centrifugation, PCR amplification, and dot blot screening. Among 2000 clones from the subtracted cDNA library, one clone with a full-length HCC-related cD...